More, we evolve new orthogonal Ribo-T/mRNA pairs that function in parallel with, but independent of, pure ribosomes and mRNAs, escalating the efficiency of orthogonal protein expression. The Ribo-T with optimized patterns is able to synthesize a various list of proteins, and may include multiple non-canonical amino acids into synthesized polypeptides. https://seymourb356nli5.cosmicwiki.com/user