The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products had been digested and cloned into a pET21a+ vector making use of NdeI and XhoI restriction enzymes. A degree mutation was launched by the https://paulp887cnx0.oneworldwiki.com/user